The lipid mobilized by anterior pituitary extract.
نویسندگان
چکیده
If filtration is required, sintered-glass funnels should be used as filter paper removes some of the dye by adsorption. Sodium bromide is used in preference to sodium chloride or potassium chloride because the detergent solution gradually develops a turbidity (not visible in the presence of the dye) on addition of the chlorides, and precipitates are formed on storage in mixtures of detergent, alkali chloride (especially potassium chloride) and Evans blue. Sodium bromide is free from this disadvantage. Commercial sodium bromide usually contains moisture and should be dried at 105-1 100 before weighing. The figure given for Stergene and Lissapol N refers to the commercially available syrupy liquid which contains about 70% water. As Triton X-100 and Triton A-20 are practically water-free, 0-3 g. of either of these detergents is equivalent to 1 g. of the other two. Evans blue, widely used for blood volume determinations (Dawson, Evans & Whipple, 1920), and first recommended for manometer fluids by Bain (1945), has proved more stable than other dyes tested. It is not taken up by the rubber or plastic reservoirs. The new fluid has a much greater cleansing power than Brodie's solution. It keeps better than the older fluid which sometimes becomes viscous on storage owing to changes in the bile salts. Froth and bubbles formed in the manometer capillary disappear more readily than from Brodie's solution. The greater cleansing power was assessed by placing in the manometer fluid a grease-coated microscopic slide prepared by dipping it into ether containing 1 % lanolin.
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ورودعنوان ژورنال:
- The Biochemical journal
دوره 48 2 شماره
صفحات -
تاریخ انتشار 1951